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The excess mortality risk in females is likely driven by either presence or predominance of excess alcohol intake over the metabolic factors alone

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A fragment of the 28S rRNA gene was amplified from 0.5 μl DNA extract using the LS1/LR5 primer pair 69,70 in a touchdown PCR procedure (94 °C for 2 min, 13 cycles of 94 °C for 1 min, 68 °C for 1 min (−0.7 °C/cycle), 72 °C for 1 min, and 26 cycles of 94 °C for 1 min, 58 °C for 1 min, 72 °C for 1 min and a final step of 72 °C for 6 min) resulting in an ∼900 bp amplicon

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