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doi: 10.1007/s11274-022-03408-z
effervescent-tablets
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Our RT-independent ligation-based assay revealed that WT ORF2p and EN-deficient mutant versions of ORF2p, but not an ORF2p RT-deficient (D702A) mutant, can remove 3’ blocking groups from the T4+AAddC and T4+3T*invT oligonucleotides, thereby allowing them to be ligated to a 5’ phosphorylated oligonucleotide ( Fig
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