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In macrophages, an exposed methionine residue (Met44) on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is identified to have critical roles in controlling the inflammatory activation ( Figure 3 )
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Its entire activity profile derives from a single primary target — NNMT inhibition — which triggers a cascade of downstream metabolic effects: An important distinction: 5-Amino-1MQ does not raise NAD+ by supplying an external NAD+ precursor, as NMN or NR do
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