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Pathway analysis indicates that the significant glycated mitochondrial proteins include essential regulators of the TCA cycle, fatty acid metabolism, and, crucially, OXPHOS, including F 1 F 0 -ATP synthase rotation and quinone binding

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Bacteria were washed twice with PBS before adding 50 μl bacterial suspensions and 50 μl of peptide (final 25 μM for 22 , 1 μM for 23 and 1 μM for 24 ) to a 96-well plate for flow cytometry (OD 595 of 1) and an 18-well IBIDI chamber pre-coated with poly-d-Lysine for imaging experiments (OD 595 of 0.25)